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Figure 5


Fig. 5. Expression of kinase-dead MLCK activates caspase-3 in MCF-10A cells. (A) Normal and Ras-transformed MCF10A cells transfected with a kinase-dead MLCK undergo apoptosis. Cells were grown on coverslips, transfected with a FLAG-tagged kinase-dead non-muscle (NM MLCK KD) or kinase-dead smooth muscle MLCK (SM MLCK KD) using Lipofectamine 2000 (Invitrogen). Cells were grown for 24 hours, fixed in 3% paraformaldehyde and stained with anti-FLAG polyclonal antibody (Sigma), Topro-3 and anti-actin (Rhodamine-phalloidin).





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