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Figure 2


Fig. 2. Localization of WAK-GFP and pectin. Pectin was detected using Jim5 and cy3 anti-rat IgG serum (red) and WAKGFP by GFP fluorescence (green), and visualized by confocal microscopy. Jim5 (No WAKGFP): single optical section of protoplast not expressing WAK-GFP but stained with Jim5. Cells transformed with WAK1-GFP were fixed after 36 hours incubation, and stained with anti-pectin Jim5 antibody, and visualized by confocal microscopy. Jim5 (WAKGFP), pectin signal alone; WAKGFP, GFP signal alone; Jim5 and WAKGFP, merged signals; Jim5 and BAKGFP, cells transformed with BAK-GFP were fixed after 5 hours incubation, and stained with anti-pectin Jim5 antibody. Magnification, x450.





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