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Figure 3


Fig. 3. WAK1-GFP colocalizes with Golgi markers. Protoplasts were transformed with WAK-GFP (green) and the indicated RFP fusion (SYP31 and SYP41, Golgi markers; SRC2, protein-storage-vacuole marker; {Delta}TIP, lytic and central vacuole marker; ARA7, endosomal marker) or stained with FM4-64 (endocytotic marker, red). Single optical sections were observed by confocal microscopy. Top two rows show single-channel fluorescence and merged images, bottom two rows show merged images only. Chlorophyll is false-colored blue. Magnification, x450.





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