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Figure 6


Fig. 6. Detection of Triton-X-100-soluble and -insoluble WAK1-TAP in protoplasts. Equal amounts of protoplasts transformed with WAK-TAP after 1, 2 or 3 days (as indicated) were extracted with 1% Triton X-100, centrifuged at 10,000 g for 5 minutes, and supernatant (S) and pellet (P) were boiled in loading buffer and either run on an SDS-PAGE (western blot) or (in a separate experiment) were slot-blotted onto nitrocellulose (slot blot). Then, both were probed with anti-TAP serum. pSmGFP, cells transformed with plasmid expressing only GFP.





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