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Fig. 7. mur1-1 does not influence WAK1-TAP insolubility. Equal amounts of wild-type (col+) and mur1-1 cells transformed with WAK1-TAP after 1 or 3 days (as indicated) were extracted with 1% Triton X-100 and centrifuged at 10,000 g for 5 minutes. The supernatant was run in an SDS-PAGE (A), or (in a separate experiment) the supernatant and the pellet were boiled in gel loading buffer and slot-blotted onto nitrocellulose (B). Pellet and supernatant were then both probed with anti-TAP serum. isx; 2.5 µM isoxaben added for 1 or 3 days. Wild-type cells were also transformed with GFP alone (GFP).
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