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Figure 2


Fig. 2. Effect of hypoxia (1% O2 for 10 minutes) on the inhibition of cellular respiration by exogenous CO in HEK293 cells. (i) Tracing of O2 consumption of control cells incubated in hypoxia. (ii) Tracing of O2 consumption of cells treated with 20 µM CO under ambient O2 concentrations. (iii) Tracing of O2 consumption of cells treated with 20 µM CO in hypoxia. No difference was observed between untreated control cells kept under ambient O2 concentrations and those incubated in hypoxia. The Ki of CO for cells in hypoxia was calculated to be 0.35 µM. Tracings are representative of at least three independent experiments.





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