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Figure 5


Fig. 5. Endogenously produced CO does not activate the p38 MAPK pathway in HEK293-HO-1 cells. (A) Phosphorylation of p38 (phospho-p38) was observed following treatment with GSNO (1 mM for 8 hours) and anisomycin (25 µg/ml for 20 minutes). (B) p38 phosphorylation was not observed in our induced HEK293-HO-1 cells after 24 hours in either ambient or hypoxic conditions; Tet, tetracycline. Blots are representative of at least three independent experiments.





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