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Figure 7


Fig. 7. The synthesis of NO is inhibited by hypoxia. (A) The production of NO by RAW264.7 cells activated for 24 hours in hypoxia was inhibited by 85%, as determined by measurement of its metabolite nitrite (NO2-). (B) The synthesis of NO decreased from a concentration of 2.3 µM at 100 µM O2 to 0.3 µM at 10 µM O2 (indicated by dashed arrows). The Km of iNOS for O2 in RAW264.7 cells was between 20 and 30 µM (indicated by the dotted arrows). n=3. *P<0.01 compared with cells activated at 1% O2.





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