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Figure 6


Fig. 6. Atonal-related bHLHs repress Nurr1-induced transactivation of TH promoter activity. Promoter assays using the reporter vectors driven by TH6.0 (A) and NL3 (B) were performed as described in Materials and Methods. The data represent mean percentages (± s.e.m.) of luciferase activities relative to that of the control (sample transfected with pEF1{alpha}-Nurr1 plus pEF1{alpha}-EGFP). Titles of the treatments including the bHLH mutants and chimeras were abbreviated as shown in Fig. 5. Luciferase activity was determined at day 2 of post-transfection and normalized to the activity of the ß-galactosidase. *Significantly different from the control at P<0.001 in the duplicates of three independent experiments.





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