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Figure 8


Fig. 8. Cell surface targeting of HA-GLUT8 and YTRF mutant in HeLa cells. Cells were transfected with plasmids for HA-GLUT8, LL/AA and YTRF mutant, and cultured for 4-6 days. The expression of the HA-GLUTs was determined by western blot using an anti-HA antibody (inset). In parallel, cell-surface levels of the HA-GLUTs were determined using an antibody binding assay as described in the Materials and Methods. Results are the means ± s.d. of three independent experiment performed in duplicate to quadruplicate.





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