QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 2. Chromator and JIL-1 immunoprecipitation assays. (A) Immunoprecipitations (ip) of lysates from S2 cells were performed using Chromator antibody (mAb 6H11, lane 4) and JIL-1 antibody (Hope antiserum, lane 3) coupled to immunobeads or with immunobeads only as a control (lane 2). The immunoprecipitations were analyzed by SDS-PAGE and western blotting using JIL-1 pAb for detection. JIL-1 antibody staining of S2 cell lysate is shown in lane 1. JIL-1 is detected in the JIL-1 and Chromator immunoprecipitation samples as a 160 kDa band (lane 3 and 4, respectively) but not in the control sample (lane 2). (B) Immunoprecipitations of lysates from S2 cells were performed using Chromator antibody (mAb 6H11, lane 3) and JIL-1 antibody (Hope antiserum, lane 4) coupled to immunobeads or with immunobeads only as a control (lane 2). The immunoprecipitations were analyzed by SDS-PAGE and western blotting using Chromator mAb 6H11 for detection. Chromator antibody staining of S2 cell lysate is shown in lane 1. Chromator is detected in the JIL-1 and Chromator immunoprecipitation samples as a 130 kDa band (lane 4 and 3, respectively) but not in the control sample (lane 2). The relative migration of molecular size markers are indicated in kDa. (C) Immunoprecipitations of nuclear extracts from S2 cells were performed using V5 antibody from cells transfected with a V5-tagged full-length Chromator (lane 2) or from untransfected cells as a control (lane 3). The immunoprecipitations were analyzed by SDS-PAGE and western blotting using JIL-1 antiserum for detection. JIL-1 antibody staining of S2 cell nuclear extract is shown in lane 1. JIL-I is detected as a 160 kDa band in V5-antibody immunoprecipitates from V5-tagged Chromator transfected S2 cells (lane 2) but not in the untransfected control samples (lane 3).

Return to article