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Figure 7


Fig. 7. RNAi depletion of Chromator in S2 cells does not affect JIL-1 chromosome localization. (A) Triple labelings with the JIL-1 pAb Hope (green), anti-MSL-1 antibody (red) and Hoechst 33258 (blue) of Chromator dsRNA treated S2 cells (lower panel) and mock-treated control cells (upper panel). The composite image (comp) is shown to the left and the location of the X chromosome is indicated with an X. (B) Western blot with Chromator mAb 6H11 of control-treated and Chromator RNAi-treated S2 cells from the cultures shown in A. In the RNAi sample Chromator protein levels (Chro) was substantially reduced compared with the level observed in the control cells. Tubulin levels (tub) are shown as a loading control.





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