spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. The N-terminal PEST sequence-containing region of SCD1 is responsible for the proteasomal degradation. (A) The PEST sequence regions in the N-terminal segment of SCD1 predicted by the PEST-Find algorithm (shown in red characters). (B) CHO-K1 cells were transfected with plasmids carrying SCD1-HA cDNA or {Delta}66 SCD1-HA cDNA and grown for 48 hours. The cells were lysed with SDS buffer and analyzed by SDS-PAGE and subsequent immunoblotting with anti-HA antibody. (C) Indirect-immunofluorescence microscopy. Fluorescence images of SCD1-HA and {Delta}66 SCD1-HA (green) and calnexin (red) were taken using a confocal microscope. Merged images are also shown. Bar, 10 µm. (D) The cells obtained above were subjected to pulse-chase analysis with [35S]methionine in the presence of DMSO or 2 µM epoxomicin. At the indicated time points, proteins were immunoprecipitated using anti-HA antibody, resolved by SDS-PAGE, and analyzed using a BAS2500 Image Analyzer. Band intensities were quantified by Image Gauge and are shown as the percentage of the signal at time zero. The results shown are the average of three independent experiments.





Right arrow Return to article