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Figure 6


Fig. 6. The 66-residue N-terminal segment of SCD1 appended to a soluble reporter protein fails to stimulate degradation. (A) CHO-K1 cells were transfected with the plasmids harboring the indicated cDNAs, grown for 48 hours, and subjected to immunofluorescence microscopy. Fluorescence images of EGFP (green) and calnexin (red) were taken using a confocal microscope. Merged images are also shown. Bar, 10 µm. (B) The cells grown as above were subjected to pulse-chase analysis. Proteins were immunoprecipitated by anti-EGFP antibody. Other conditions were as described in Fig. 5D. (C) CHO-K1 cell were transfected with EGFP, SCD66-EGFP, SCD33-EGFP, or SCD1wt-EGFP constructs. After 48 hours, the transfected cells were treated with cycloheximide (10 µg/ml) for 0, 2, and 4 hours and their fluorescence was determined using flow cytometric analysis. The results shown are the average of three independent experiments.





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