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Figure 1


Fig. 1. RNAi of Klp10A diminishes the degree of apoptosis associated with Orbit depletion. (A) Knockdown of orbit alone (pink line) decreases the number of 2N cells while slightly increasing the number of presumptive mitotic cells with a 4N DNA content and dramatically increasing the number found in apoptosis relative to controls (solid green). (B) Klp10A downregulation (blue line) reduces the number of 2N cells while lowering and broadening the 4N peak, suggesting aneuploidy. (C) Double RNAi of orbit and Klp10A (black line) decreases the number of apoptotic cells seen following orbit RNAi as well as slightly increasing the proportion of 2N cells. Also shown are the relative distributions of cell population following each of the indicated treatments. (D) Western blot analysis of cell extracts taken from dsRNA-treated cells reveals substantial protein knockdown 72 hours after treatment. Actin is the loading control.





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