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Figure 5


Fig. 5. Fluorescence microscopy of microtubule and actin distribution in apoptotic A431 cells. Confocal (A-C) and wide-field (D) fluorescence images of cytospin preparations of floating, UV-irradiated apoptotic A431 cells. (A,B) Apoptotic cells labelled with phalloidin (F-actin; green), anti-tubulin antibody (red) and DAPI (blue). (B') Detail of a cluster of apoptotic bodies formed at the tip of two spikes from the boxed region of the panel above. (C) Actin and microtubules co-align in apoptotic spikes. The body of the cell is located to the top left. (D) An isolated apoptotic body labelled for tubulin (red), cleaved PARP (green) and DAPI (blue). Bars, 10 µm (A,B); 2 µm (B',C); 5 µm (D).





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