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Fig. 6. Microtubule and chromatin dynamics in apoptotic A431 cells. (A) Time-lapse imaging of anisomycin-treated A431 cells transiently co-expressing YFP-tubulin (green) and HMGB1-CFP (red). Alexa Fluor 594-Annexin V labelling is false-coloured blue. Fluorescence frames are from supplementary material Movie 6. Bar, 20 µm. (B,C) Zoomed areas from the boxed regions indicated in A showing increased temporal resolution (annexin V channel omitted). Arrowheads indicate packets of condensed chromatin. Bar, 10 µm.