spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 8


Fig. 8. Orientation and dynamics of apoptotic microtubules. (A) Confocal immunofluorescence imaging of EB1 distribution in an apoptotic A431 cell. EB1 puncta (red) are localised to the distal tips of microtubules (green) within apoptotic spikes. The boxed areas of the merge image are shown in close-up to the right. Bar, 10 µm. (B) Kymograph derived from a time-lapse sequence of EB1-GFP dynamics in an apoptotic A431 cell spike (supplementary material Movie 7). Puncta (arrows) move towards the spike tip. (C) FRAP analysis of microtubule polymer turnover in apoptotic spikes. Frames represent images of apoptotic spikes from A431 cells stably expressing YFP-tubulin, before and after photobleaching (boxed areas). To the right, quantification of fluorescence recovery in the boxed region. Bar, 10 µm.





Right arrow Return to article