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Fig. 2. Calpain regulates podosome disassembly in DCs. (A) Calpain activity in the presence of inhibitors was detected. As expected, inhibition of calpain activity was found using the calpain inhibitors ALLM (50 µM), ALLN (50 µM) or the calpastatin peptide (Calp Pept, 50 µM) but not using cathepsin inhibitor 1 (Cathep. Inh,10 µM), proteosome inhibitor 1 (Proteo. Inh, 10 µM) or the scrambled version of the calpastatin peptide (Calp Pept NC, 50 µM). (B) The percentage of DCs with podosomes was not affected by treatment with calpain inhibitors. Inhibition of cathepsin B and proteosome resulted in DC podosome loss. Results are mean values ± s.e.m. from 50 cells with three coverslips examined per experiment. *P<0.05 and ***P<0.005 compared with values in untreated cells (Student's t-test).
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