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Fig. 9. WASP is required for formation of dynamic DC podosomes and location of WASP in DC is regulated by calpain activity. WASP-null DCs expressing eGFP-WASP were plated on poly-L-lysine-coated coverslips overnight and then left untreated (A,B) or treated with the calpain inhibitor ALLM (C,D) and mounted in viewing chambers to measure adhesion turnover index using confocal microscopy. Micrographs of the interference reflection signal (A,C) and eGFP-WASP location (B,D) were taken 20 seconds apart. Bars, 10 µm. (E) Quantification of adhesion turnover of WASP-null DCs expressing eGFP constructs in the presence or absence of calpain inhibitors. The graphs illustrate the mean percentage of cells ± s.e.m. of 20 cells per treatment group from three experiments. Significant differences were observed at *P<0.05 and **P<0.01 as indicated.
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