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Figure 5


Fig. 5. GST affinity chromatography of snapin with native RyR. GST pull-down experiments of native RyR incubated with purified GST fusion proteins and detection by western blotting. Solubilised (A) skeletal muscle (200 µg) or (B) cardiac heavy SR (1 mg) were incubated with purified GST fusion proteins (2 nmol) as indicated, proteins were precipitated with glutathione-Sepharose 4B beads, and co-precipitated RyR was analysed by western blotting using Ab2149 (4% SDS-PAGE/agarose gels). The first two lanes show loading of (A) skeletal muscle or (B) cardiac heavy SR.





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