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Figure 5


Fig. 5. CP orientation in {gamma}-tubulin, PACRG and PFR2 RNAi mutant cell lines. (A) The remaining CP microtubule in 9+1 axonemes resulting from {gamma}-tubulin ablation has a random orientation. (B) Knockdown of PACRG severely disrupts outer doublet microtubules, but only partially removes CP constraint in X+2 axonemes. (C) Immotility as a result of PFR2 ablation does not affect CP orientation. (D) Radial distribution of the doublets and the CP microtubules in transverse sections of axonemes from the induced {delta}-tubulin and PACRG RNAi trypanosomes.





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