spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Endosomal clathrin concentrates Hrs in EEA1-negative microdomains. (A,B) HeLa-cells were co-transfected with plasmid-based clathrin-specific siRNA and Rab5Q79L, and stained with anti-clathrin, anti-Hrs or anti-EEA1 antibodies for immunofluorescence confocal microscopy. (A) Control cell with endosomal clathrin. (B) siRNA-transfected cell where clathrin is efficiently knocked down. Yellow indicates colocalisation of Hrs and clathrin, and cyan indicates colocalisation of Hrs and EEA1 in the merged images. Insets show enlarged endosomes. The amount of endosomal Hrs that colocalise with EEA1 was quantified as described in Materials and Methods (C). Error bars give the mean ± s.e.m. Control, n=25; siRNA clathrin, n=27. (D,E) HEp-2 cells were transfected with Hrs-specific siRNA oligonucleotides. The siRNA-treated cells were transiently co-transfected with Rab5Q79L and myc-Hrswt (D) or myc-Hrs{Delta}CB (E). Insets show two different enlarged endosomes from each transfection. Yellow indicates colocalisation of Hrs and EEA1. Bars, 10 µm. (F) The amounts of endosomal myc-Hrswt or myc-Hrs{Delta}CB that colocalised with EEA1 were quantified. Error bars give the mean ± s.e.m. Myc-Hrswt, n=14; myc-Hrs{Delta}CB, n=14.





Right arrow Return to article