spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. Membrane-associated Hrs and clathrin are rapidly exchanged with cytosolic pools. HeLa cells were co-transfected with Rab5Q79L and GFP-clathrin LC or YFP-Hrs, and studied live at 25°C as described in Materials and Methods. After photobleaching of whole endosomes, both Hrs and clathrin recovered in microdomains on the limiting membrane of the enlarged endosomes. (A) Representative images of GFP-clathrin LC and YFP-Hrs. (B) Representative recovery curve for YFP-Hrs. (C) Representative recovery curve for GFP-clathrin LC. The half time of the recovery, t1/2, was calculated from several recordings. YFP-Hrs, n=12; GFP-clathrin LC, n=9.





Right arrow Return to article