spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. Response of chicken INK4b and ARF to different signalling pathways. (A) CEFs (32-38 PDs) were infected with retroviruses encoding an activated form of human H-Ras (Ras), human E2F1 or SV40 T-antigen (T-Ag), with corresponding empty vector controls (Vec). Following drug selection, the cells were analysed for expression of the exogenous Ras, E2F1 and T-Ag by immunoblotting (B) Samples of total RNA were analysed for the expression of INK4b and ARF RNA by northern blotting, using probes specific for the 3'-untranslated region of each transcript. GAPDH was used to control for loading. (C) CEFs were infected with MC29 virus or MH2 virus as indicated and total RNA was prepared at 2 days post-infection. Expression of INK4b and ARF was assessed by northern blotting, and virus infection was confirmed using a probe representing the entire ALV genome.





Right arrow Return to article