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Figure 7


Fig. 7. Chicken INK4b is expressed in post-mitotic cells in the roof-plate of rhombomere 1. (A-D) Whole-mount in situ hybridisation for INK4b in chicken embryos at stage 13 (B), stage 17 (A,C) and stage 20 (D). The expression, confined to r1, is detected at least until stage 24. (B-D) Magnifications of the hind-brain region. Anterior is at the top of the figure. (E-G) Roof-plate cells expressing INK4b are post-mitotic. Cross-section from an embryo at stage 24 was hybridised in situ with the chicken INK4b probe (red) and stained with anti-phospho-histone H3 antibody (green). INK4b expression is in the roof-plate. (F,G) Higher magnifications of a part of E, showing that roof-plate cells, expressing INK4b are not stained with anti-phospho-histone H3 antibody (p-H3); nuclei were detected with TO-PRO-3 iodide (TO-PRO). (G) The green and red channel of F. Note that there is no overlap between green and red signals. The arrowheads indicate the expression. Dorsal is up. rp, roof-plate; fp, floor-plate; r1, rhombomere 1; r3, rhombomere 3; ov, otic vesicle. Bars: A and D, 1 mm; B,C,E-G, 0.2 mm.





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