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Figure 5


Fig. 5. GAG chains are not required for filopodial extension or complete B82L cell spreading. B82L cells were detached using EDTA and treated in suspension either without (A,C,F,I) or with a combination of heparinases I and III and chondroitin ABC lyases (B,D,G,J) for 2 hours before plating on 200 µg/ml HBD-FN (A,B), 60 µg/ml mAb 281.2 (C,D), 60 µg/ml plus 1 µg/ml VN (F,G) or 5 µg/ml VN (I,J). Quantification of treated (white bars) or untreated (gray bars) cell extension of filopodia on 60 µg/ml mAb 281.2 (E) or complete cell spreading on mAb 281.2 supplemented with 1 µg/ml VN or 3 µg/ml FN (H) or complete cell spreading on 5 µg/ml VN or 60 µg/ml FN (K) is also shown. Bar, 50 µm.





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