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Fig. 6. B82L-cell spreading on VN is blocked by recombinant S1ED. (A) B82L fibroblasts were plated on 5 µg/ml VN in the absence of other treatment, or in the presence of 30 µM GST, 1-30 µM GST-mS1ED or 30 µM GST-mS4ED (inset), then fixed and stained with Rhodamine-phalloidin for visualization. (B) Quantification of cell adhesion in triplicate samples (± s.e.) plated either with no additions, or concentrations of GST-mS1ED ranging from 0-30 µM. Bar, 50 µm.