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Figure 4


Fig. 4. Effect of LMB on nuclear p53 exclusion in HCMV-infected HUVECs. The infected cells were treated with 20 nM LMB for 18 hours before observation. LMB was able to abrogate the nuclear exclusion when the cells were treated before the onset of nuclear-p53 exclusion (arrows, 4 dpi). Once p53 was completely exported out of nucleus (5 dpi), LMB was unable to inhibit nuclear exclusion. Cells were stained with anti-p53 (red, Texas Red-X), anti-HCMV-IE proteins (green, FITC) and DAPI (blue) to counterstain the nuclei.





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