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Fig. 8. MG132 treatment did not cause any significant changes in p53 and Mdm2 patterns or subcellular localization in HCMV-infected HUVECs. HCMV-infected cells (5 dpi at MOI=0.3) were treated with or without MG132 for 6 hours before IFA. All IFA images were taken with the identical cells conditions, level of infections, IFA protocol, and equal exposure/digital picture processing conditions. Cells were stained red by the anti-p53-Texas Red-X, green by anti-Mdm2-FITC and blue by DAPI for nuclear counterstaining. MG132 treatment had no effect on the subcellular localization of p53. However, a slight increase of both p53 and Mdm2 levels can be observed after MG132 treatment compared to cells without treatments. Stars indicate the uninfected cells, which have relatively low levels of p53 and Mdm2 in the nuclear region. Mdm2 and p53 levels were significantly increased, mainly in the nuclear region of the infected cells at very early stage of post-infection (white arrows). During the period of p53 nuclear exclusion (white arrows `a') the p53 and Mdm2 levels were elevated mainly in the nuclear and also in parts of the cytoplasmic regions. At the later stages of infection, cytoplasmic p53 sequestration was observed coincidentally with the lower levels of Mdm2 (yellow arrows).
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