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Figure 9


Fig. 9. Effect of CsA on distribution of VF and MF, and phosphorylation of AQP2. (A) IMCD-cell VFs were isolated from control and CsA-treated mice. Expression of total and p-AQP2 was detected by immunoblotting with specific antibodies. Tubulin was included as a control. Lanes 1-3, control; lanes 4-7, CsA-treated mice. Levels of total and p-AQP2 were evaluated by semi-quantitative analysis and data from 3-5 mice are shown as the mean ± s.e.m. *P<0.05 (Student's t-test). (B) MFs were prepared and expression of total and p-AQP2 was detected by direct immunoblotting. Tubulin was included as a control. Lanes 1-4, control; lanes 5-8, CsA-treated. Levels of total and p-AQP2 were evaluated by semi-quantitative analysis and data from 3-5 mice are shown as the mean ± s.e.m. *P<0.05, **P<0.01 (Student's t-test).





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