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Figure 3


Fig. 3. Expression of candidate effectors by human MPs and mpcs. (A) RT-PCR analysis of CX3CL1, VCAM-1, ICAM-1 and PECAM-1 mRNA in MPs, and of CX3CR1, {alpha}4, {alpha}L, ß2 integrins, and PECAM-1 mRNA in mpcs. ß2M is beta2microglobulin. (B) Immunolabelling of CX3CL1, VCAM-1, ICAM-1 and PECAM-1 on MPs (left panel) and of CX3CR1, VLA-4 and LFA-1 on mpcs (right panel), revealed by DAB substrate kit for peroxidase. Magnification, 300x. PECAM-1 expression in mpcs was assessed by immunoblotting. MT, myotube; Mb, myoblast.





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