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Figure 7


Fig. 7. Prediction and determination of PEX19-binding sites in S. cerevisiae Pex15p. (A) Graphic representation of the mPTS of Pex15p as proposed by Elgersma et al. (Elgersma et al., 1997). Shown are the TMD as well as the proposed peroxisomal (Px-TS) and ER (ER-TS) targeting signal sequences. (B) Location of predicted PEX19-binding sites in the mPTS of yeast Pex15p. The two PEX19-binding sites as predicted by our program (Rottensteiner et al., 2004) are denoted. (C) Yeast two-hybrid assay for the interaction of Pex15p with Pex19p. The indicated fragments of Pex15p were tested as GAL4-BD fusions in combination with yeast Pex19p fused to the GAL4-AD (left panel) or the GAL4-AD alone (right panel) in the yeast strain PJ69-4a. Two independent colonies of each transformation were assessed for growth on histidine-adenine double-dropout medium plates.





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