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Figure 9


Fig. 9. Peroxisomal redirection of the mitochondrial TA protein Fis1p by the luminal PEX19-binding site of Pex15p. A GFP fusion of Fis1p was coexpressed in the S. cerevisiae wild-type strain with the synthetic mitochondrial marker PrF0ATP9-DsRed (Mc, A) or PTS2-DsRed (Px, B). GFP- and DsRed-derived fluorescence was analyzed after strains had been grown on ethanol-containing plates for 2 days. Localization of GFP-Fis1p-Pex15p361-383 was similarly determined. DIC, differential interference contrast. Bar, 2 µm.





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