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Fig. 1. RB-like structures form in the absence of L chains when µ ${Delta}$ CH1 synthesis is increased. (A) Induction of RB in myeloma transfectants. N[µ ${Delta}$ CH1], N[µ1] and J[µ ${Delta}$ CH1] cells were treated with or without 1 mM sodium butyrate (NaBut) for 40 hours, fixed and stained with fluorescent anti-µ antibodies. Note that RB-like structures are induced by NaBut treatment in N[µ ${Delta}$ CH1] but not in N[µ1]. As previously reported (Valetti et al., 1991), RB are present also in untreated, ${lambda}$ producing J[µ ${Delta}$ CH1] cells; bar, 5 µm. (B) The presence of RB correlates with accumulation of detergent-insoluble µ ${Delta}$ CH1 chains. Cells were lysed in TX100 and equal amounts of soluble (s) and insoluble (i) fractions resolved under reducing conditions, blotted and decorated with anti-µ. (C,D) Different RB morphology in myeloma cells is dependent on L chain synthesis. J[µ ${Delta}$ CH1] and N[µ ${Delta}$ CH1] cells, the latter treated with 1 mM NaBut for 40 hours, were processed for both Epon embedding (C) and cryo-sectioning (D). Ultrathin cryo-sections were immuno-gold labeled using anti-µ antibodies and protein A-gold (15 nm). Arrowheads point to membrane-associated ribosomes. Bar, 100 nm. (E) The table summarizes the RB diameters (expressed in µm0 in N[µ ${Delta}$ CH1] cells treated with 1 mM NaBut for 40 hours, and in untreated J[µ ${Delta}$ CH1] cells. Measurements were carried out on electron microscope images. 60 and 30 RB were measured in N[µ ${Delta}$ CH1] and J[µ ${Delta}$ CH1] cells respectively, each from two independent preparations.

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