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Fig. 4. Drosophila vha55 complements ${Delta}$ vma2 in yeast. The Drosophila vha55 gene was cloned into in plasmid p415 met 25 (low copy, left), or p425 met 25 (high copy, right). 1, empty vector in wt yeast; 2, vma2 in wt yeast; 3, vha55 in wt yeast; 4, vha55::GFP construct in wt yeast; 5, vha55-ORF construct in wt yeast; 6, vha55::GFP in ${Delta}$ vma2 strain; 7, vha55-ORF in ${Delta}$ vma2; 8, vha55 in ${Delta}$ vma2; 9, VMA2 in ${Delta}$ vma2; 10, empty vector in ${Delta}$ vma2. (The upper panel plates were permissive: Leu^-, Met^-, pH 5.0; the lower panel plates were restrictive: Leu^-, Met^-, pH 7.5. Only yeast with functional V-ATPase can grow at pH 7.5.)

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