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Figure 3


Fig. 3. Unlike CED-9, Bcl-2 does not alter CED-4 localisation in yeast. Yeast were transformed with the indicated plasmids. Transformants were either stained for nucleic acid using DAPI (blue; upper three rows) or mitochondria using MitoTracker (red; middle three rows). The endoplasmic reticulum was visualised by anti-Kar2p immunofluorescence (red; lower three rows). GFP-tagged CED-4, CED-9 and Bcl-2 were detected (green fluorescence). GFP-CED-4 localisation was also observed in the presence of either FLAG-CED-9 or Bcl-2. Bars, 5 µm.





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