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Figure 2


Fig. 2. Effect of AZI on proliferation of non-transformed NIH-3T3 fibroblasts. (A) Western blot analysis of 50 µg of total cell lysate from NIH-3T3 cells stably infected with retroviral vectors pWZL or pWZL-AZI. (B) Proliferation of control ({diamond}) and AZI-overexpressing NIH-3T3 cells ({square}) grown in low serum (0.5% BCS). Cells were plated at a density of 5x104 cells in 12-well plates and the total number of cells per well were counted. Error bars indicate s.e.m. (C) To assess saturation density, control ({diamond}) and AZI-overexpressing NIH-3T3 cells ({square}) were plated at 1x104 cells in six-well plates in media containing 10% BCS and cell number assessed over 11 days.





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