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Figure 3


Fig. 3. AZI expression increases ODC activity in AT2.1 and NIH-3T3 cells. (A) ODC activity (pmol 14CO2/mg protein/hour) was measured in control and AZI-overexpressing AT2.1 cells, using [1-14C]-L-ornithine as the substrate. Total cell lysates were collected 24 hours after plating 5x105 cells in medium containing 10% serum. Error bars indicate s.e.m. (B) Effect of AZI overexpression on cellular ODC activity in NIH-3T3 fibroblasts. ODC activity was measured as above. (C) Polyamine uptake by AZI-overexpressing cells was measured following a 15 minute incubation with 0.25-10 µM [14C]spermine, as described in the Materials and Methods. No significant difference was observed in spermine uptake between AT2.1 vector control ({diamond}) and AZI-overexpressing cells ({square}). Error bars indicate s.e.m.





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