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Fig. 5. Overexpression of AZI_117-140 increases proliferation of AT2.1 cells without increasing ODC activity in AT2.1 cells. (A) Proliferation of AT2.1 cells stably transfected with pTriEx-Hygro, pTriEx-Hygro-AZI, or pTriEx-Hygro-AZI_117-140, was measured as described in Materials and Methods, in media containing 1% FBS. Error bars indicate s.e.m. , Vector control AT2.1 cells; , AZI-overexpressing AT2.1 cells; , AZI_117-140 overexpressing AT2.1 cells. The proliferation rate of AZI_117-140 overexpressing cells decreased compared with wild-type AZI-overexpressing cells, but still demonstrated increased proliferation relative to vector controls. An average doubling time of 22.8±1.6 (s.d.) for control cells; 15.6±1.7 for AZI-overexpressing cells; and 18±1.7 for AZI_117-140 overexpressing cells was determined from two separate experiments. (B) ODC activity was measured in control, AZI and AZI_117-140-overexpressing AT2.1 cells. 5x10⁵ cells were plated in RPMI supplemented with 10% FBS, and ODC activity assessed as described in the Materials and Methods. Error bars indicate s.e.m.

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