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Figure 7


Fig. 7. AZI shows ability to attenuate cyclin D1 degradation. Cyclin D1, wild-type AZI and AZI{Delta}117-140 were synthesized by in vitro transcription and translation in separate reactions. All three proteins were radiolabeled with [35S]methionine. Translated proteins were combined and incubated in an ATP-regenerating buffer for 0, 30, 60, 120 and 180 minutes. The amount of cyclin D1 remaining at the indicated time points was assessed by SDS-PAGE and PhosphorImager analysis (lower panel). Results are representative of two independent experiments. bullet, cyclin D1 alone; {blacktriangleup}, Cyclin D1 + wild-type AZI; {blacksquare}, % Cyclin D1 + AZI{Delta}117-140.





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