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Figure 2


Fig. 2. Time course of TRPL-eGFP translocation. At the indicated time points fluorescence images of intact eyes of flies expressing TRPL-eGFP (yw; trpl-eGFP) were obtained using the water immersion technique. From these images the percentage of TRPL-eGFP present in the rhabdomeres at different times after switching the flies from darkness to light (A) or vice versa (B) was calculated (see Materials and Methods). Each data point represents the mean value ± s.d. of at least five independent measurements. (C) Dark-(dark l/d) or light-adapted flies (light d/l) expressing TRPL-eGFP (yw; trpl-eGFP) were subjected to alternating light and dark intervals of 30 minutes each for 16 hours. The percentage of TRPL-eGFP present in the rhabdomeres was determined. The values of dark- and light-adapted flies are shown additionally. Mean values ± s.d. of at least five experiments are shown.





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