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Figure 4


Fig. 4. Functional rhodopsin is required for the light-dependent translocation of TRPL-eGFP. Wild-type (A,B), yw; ninaE17; trpl-eGFP (C,D), yw/w; ninaE17/P[Rh1+3]; trpl-eGFP (E,F) and w; trpl-eGFP; ninaBP315 (H-J) flies expressing TRPL-eGFP were raised in the dark or in orange light for 16 hours. ninaE17 + Rh3 was also illuminated with UV for 16 hours (G). Representative images of the eGFP fluorescence in intact eyes obtained by the water immersion technique are shown. For light-raised ninaBP315 two individuals are shown that revealed different levels of TRPL-eGFP internalization (I,J). Bar, 15 µm. In K, dark-raised flies were transferred to white, blue, green or orange light for 16 hours. Then the percentage of TRPL-eGFP present in the rhabdomeres was determined. Mean values ± s.d. of at least five experiments are shown.





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