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Figure 5


Fig. 5. Role of arrestins in the translocation of TRPL-eGFP. Water immersion images of eGFP-fluorescence in the eyes of the following arrestin mutants raised for 16 hours in the dark or in orange light are shown: (A,B) arr11 cn bw; trpl-eGFP, (C,D) w; trpl-eGFP; arr23 st, (E,F) yw trpl-eGFP; arr11 cn bw; arr23 (arr11 arr23 double mutant). Bar, 15 µm. (G) The rhabdomeral amount of TRPL-eGFP in the dark-raised (black bars) and orange light-exposed (white bars) arrestin mutants was quantified as in Figs 4, 5. For comparison, the relative amount of TRPL-eGFP in the rhabdomeres of dark-raised and orange light-exposed wild-type flies (WT), as determined in Fig. 5, is shown. Values are expressed as a percentage of the R values of dark-raised wild-type flies.





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