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Fig. 7. Removal of extracellular Ca²⁺ inhibited light-induced translocation of TRPL-eGFP. Optical sections of live isolated retinas of flies expressing TRPL-eGFP on a null trpl background (yw; trpl-eGFP trpl³⁰²) are shown. The images were obtained by confocal microscopy using a water immersion objective (Olympus 60x/0.9 w LUMPLan F1). (A,D) Images obtained from sliced heads illuminated for 4 hours (Schott OG 590 unattenuated orange light) and incubated in oxygenated extracellular solution with 1 mM EGTA supplemented with 1% FBS and 5 mM sucrose. (B,E) Images obtained from illuminated sliced heads incubated in oxygenated extracellular solution with 1 mM Ca²⁺ supplemented with 1% FBS and 5 mM sucrose. (C) Image obtained from isolated retina of dark-raised flies. Bars, 10 µm (A,B) and 2 µm (C-E).

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