spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. Suppression activity of ArfGAPs, BET1, BOS1, OLE1, and GYP6 for arf1-16 and arf1-17 ts mutants. (A) arf1-16 (NYY16-1) and arf1-17 (NYY17-1) cells were transformed with pNY24-GLO3 (2 µ GLO3), pNY24-GCS1 (2 µ GCS1), pNY24-AGE1 (2 µ AGE1), pNY24-AGE2 (2 µ AGE2), and the transformants were grown at 23°C. Cells were diluted to a final concentration of 1.0 at OD 600, and 10 µl of 10-fold dilutions were spotted on MCD (minimal glucose casamino acid medium) (-Trp) plates. The plates were incubated at the indicated temperatures for 2 days. Integrants of wild-type ARF1 (WT, NYY0-1) and mutants transformed with pYO324 (vector) were used as controls. (B) arf1-16 and arf1-17 cells were transformed with pNY25-BET1 (2 µ BET1), pNY25-BOS1 (2 µ BOS1), pNY25-OLE1 (2 µ OLE1), and pNY25-GYP6 (2 µ GYP6), and the transformants were spotted on MVD (-Leu) plates and grown as described in A. Integrants of wild-type ARF1 (WT) and mutants transformed with pYO325 (vector) were used as controls.





Right arrow Return to article