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Figure 4


Fig. 4. Time course of Arf GAP activity of Glo3p and Gcs1p. 0.2 µM recombinant Glo3p (circle) or Gcs1p (triangle) was incubated with 1 µM [{alpha}-32P]GTP-loaded WT-Arf1 (upper panel) or arf1-17 (lower panel) protein at 23°C (closed circle and triangle) and 35°C (open circle and triangle). Aliquots were withdrawn at the indicated times and subjected to the analysis of bound nucleotide by thin-layer chromatography.





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