spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. UBF is localised next to viral replication centres. (A-C) UBF is shown in green (anti-UBF serum), DBP in red and the location of the nucleoli is revealed by phase contrast. (A) The location of UBF in uninfected HeLa cells relative to the nucleoli. (B) The location of UBF in adenovirus-infected cells at 16 hours post-infection is shown relative to viral DBP and the nucleoli. (C) Adenovirus-infected cells were examined at 8 hours post-infection when the replication centres are just beginning to form. (D) The location of preTP (in red) and UBF (in green) was examined in infected cells at 18 hours post-infection. The inset in the final merged image is a close up to illustrate that UBF and preTP staining is proximal but does not overlap in the main. All images are of a single focal plane approximately 0.3 µm depth, bar, 10 µm. (E) A western blot of equal numbers of either uninfected cells or adenovirus-infected cells after 16 hours, probed with anti-UBF antiserum to reveal total quantities of protein. The location of the molecular mass markers is shown on the left.





Right arrow Return to article