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Fig. 2. The location of different mutations of EGFP-UBF in uninfected and infected cells. (A) A schematic diagram of UBF. The top drawing indicates the relative location of the 6 HMG boxes (numbered 1-6), the dimerisation domain (cross hatching) and the transactivation domain (marked with dots). The six schematics below indicate the position of EGFP (shaded box), the relative extent of the UBF deletion mutant fused to the C-terminus of EGFP and the exact number of amino acids from UBF fused to EGFP. (B) The usual location of each fusion protein in HeLa cells at 18 hours post-transfection, B23 is in red and the EGFP fusion is in green. (C) The cells were infected with adenovirus and transfected with plasmid encoding the appropriate fusion protein. The EGFP fusion is shown in green and the location of viral DBP is shown in red. All images are of a single focal plane approximately 0.3 µm depth; bar, 10 µm.

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