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First published online June 20, 2006

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Top TIPs for membrane trafficking

Proteins that localize to the plus ends of microtubules – +TIPs – have been implicated in the capture of membranes by microtubules prior to intracellular transport. New data from Peter Watson and David Stephens now reveal the order in which these arrive and indicate that the loading of one +TIP – p150^Glued, a component of the dynactin-dynein motor complex – is not required for intracellular trafficking in mammalian cells (see p. 2758). The authors use RNAi to show that the +TIP EB1 is needed for the localization of CLIP-170 at microtubule plus ends and that this is in turn needed to maintain the localization of p150^Glued. They also show that, although removal of p150^Glued from microtubule plus ends by depletion of CLIP-170 affects several cellular processes, it does not disrupt the localization or motility of intracellular organelles. Furthermore, a GFP-p150^Glued fusion protein can be incorporated into dynactin complexes and recruited to membranes independently of its interaction with microtubule plus ends. These data, the authors conclude, argue against the idea that a pool of dynactin at microtubule plus ends is involved early in membrane trafficking.

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Microtubule plus-end loading of p150^Glued is mediated by EB1 and CLIP-170 but is not required for intracellular membrane traffic in mammalian cells

Peter Watson and David J. Stephens
JCS 2006 119: 2758-2767. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?